Application

This mouse monoclonal Anti-Lamin A/C Antibody, clone 2E8.2, Alexa Fluor^® 488 Conjugate, Cat. No. MABT538-AF488 is used for the detection of Lamin A/C by Immunocytochemistry.

Research CategoryCell Structure

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Lamin A/C in A431 cells.

The unconjugated antibody (Cat. No. MABT538) is shown to be suitable also for Flow Cytometry, Immunohistochemistry, and Immunoprecipitation applications.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Lamin-A/C (UniProt: P02545; also known as 70 kDa lamin, Renal carcinoma antigen NY-REN-32) is encoded by the LMNA (also known as LMN1) gene (gene ID: 4000) in human. Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Proteolytic cleavage of 18 residues from the C-terminal of prelamin-A/C results in the production of lamin-A/C. Proteolytic cleavage requires prior farnesylation and methylation, and absence of these blocks cleavage. The prelamin-A/C maturation pathway includes farnesylation of CAAX motif, ZMPSTE24/FACE1 mediated cleavage of the last three amino acids, methylation of the C-terminal cysteine and endoproteolytic removal of the last 15 C-terminal amino acids. Farnesylation of prelamin-A/C also facilitates nuclear envelope targeting. Lamin A and C are present in equal amounts in the lamina of mammals. Lamin plays an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Lamin C differs from canonical form A in the following manner: amino acids 567-572 are changed from GSHCSS to VSGSRR and form C does not amino acids 573-664 seen in canonical form A.

Immunogen

GST/His-tagged recombinant fragment corresponding to 109 amino acids from the C-terminal half of human Lamin A/C.

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Purified mouse monoclonal antibody in PBS with 1.5% BSA and 0.05% sodium azide.

Quality

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Lamin A/C in HeLa cells.

Specificity

Clone clone 2E8.2 specifically recognizes Lamin A/C in HeLa cells. This antibody has been shown to bind to an epitope between amino acids 464-572 in the C-terminal half.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

74.14 kDa calculated. Uncharacterized bands may be observed in some lysate(s).